Protein_Domain

Part:BBa_K2401008:Design

Designed by: Shanti Ricke   Group: iGEM17_Hamburg   (2017-10-21)


HMWP 1 High molecular weight synthase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 1839
    Illegal NgoMIV site found at 2578
    Illegal NgoMIV site found at 3085
    Illegal NgoMIV site found at 8065
    Illegal AgeI site found at 1251
    Illegal AgeI site found at 2209
    Illegal AgeI site found at 3126
    Illegal AgeI site found at 8365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 790
    Illegal BsaI.rc site found at 9309
    Illegal SapI site found at 5562
    Illegal SapI.rc site found at 190


Design Notes

The natural sequence of HMWP 1 does include multiple restriction sites that fall under RFC10 therefore we had to generate a sequence that is RFC10 compatible. Another issue was the immense length of the gene with more than 9 kb IDT was unable to synthesize it all at once so we had to introduce some RFC10 compatible single cutter restriction sites to ligate the gene together.


Source

Genomic sequence Klebsiella pneumonia

References